Phosphatidylinositol 3-kinase regulatory subunit alpha is an enzyme that in APPLICATION DATA humans is encoded by the PLK3R1 gene. Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position Immunohistochemical analysis The enzyme comprises a 110 KD catalytic subunit and a regulatory subunit of e paraffin-embedded rat brain tissue ither 85, 55, or 50 KD. This gene encodes the 85 KD regulatory subunit using Phospho-p13-kinase p85-alpha/ Phosphatidylinositol 3-kinase plays an important role in the metabolic actions gamma(Iyr467/199)pab insulin, and a mutation in this qene has been associated with insulin resistance. Alternative splicing of this gene results in three transcript variants encoding different isoforms
This Abmart polyclonal antibody is produced by immunizing animals with a synthetic phosphopeptide derived from human Pl3 kinase p85 alpha/gamma Immunohistochemistry-anti-phospho- around the phosphorylation site of(iyr467/199) Pl3-kinase p85-alpha/gamma (Tr467/199pAb(#T40116
Store at-20oc for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at-20'c When reconstituted in All lanes: Phospho-p]3-kinase p85- sterile PH 7.40.01M PBS or diluent of antibody the antibody is stable for alpha/gamma (Iyr467/199) pab at 100 1/300 dilution at least two weeks at 2-4.C
Phosphatidylinositol 3-kinase regulatory subunit alpha, Pl3-kinase regulatory subunit alpha, PI3K regulatory subunit alpha, Ptdins-3-kinase regulatory subunit alpha, Phosphatidylinositol 3-kinase 85 kda Goat Anti-rabbit IGG-HRP, 5% skim milk conjugated at 1/10000 dilution regulatory subunit alpha, P13-kinase subunit p85-alpha, Ptdlns-3-kinas regulatory subunit p85-alpha, PIK3R1, GRB 1
Human, Mouse, Rat, Dog, Pig, Cow, Horse
85 kda
Western blotting 1:500-1:2000 ELISA 1:500-1:1000 HC-P/IHC-F 1:400-1:800 1:100-1:500
PI3 Kinase p85 alpha Antibody detects endogenous levels of total PI3 Kinase p85 alpha
A synthesized peptide derived from human PI3 Kinase p85 alpha
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Store at -20°C long term.
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