兔单抗-PI3 Kinase p85 alpha Antibody

Datasheet

DESCRIPTION

Phosphatidylinositol 3-kinase regulatory subunit alpha is an enzyme that in
APPLICATION DATA
humans is encoded by the PLK3R1 gene. Phosphatidylinositol 3-kinase
phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position
Immunohistochemical analysis
The enzyme comprises a 110 KD catalytic subunit and a regulatory subunit of
e paraffin-embedded rat brain tissue
ither 85, 55, or 50 KD. This gene encodes the 85 KD regulatory subunit
using Phospho-p13-kinase p85-alpha/
Phosphatidylinositol 3-kinase plays an important role in the metabolic actions
gamma(Iyr467/199)pab
insulin, and a mutation in this qene has been associated with insulin
resistance. Alternative splicing of this gene results in three transcript variants
encoding different isoforms


SOURCE

This Abmart polyclonal antibody is produced by immunizing animals with a
synthetic phosphopeptide derived from human Pl3 kinase p85 alpha/gamma Immunohistochemistry-anti-phospho-
around the phosphorylation site of(iyr467/199)
Pl3-kinase p85-alpha/gamma
(Tr467/199pAb(#T40116


STORAG

Store at-20oc for one year. Avoid repeated freeze/thaw cycles. The
lyophilized antibody is stable at room temperature for at least one month
and for greater than a year when kept at-20'c When reconstituted in
All lanes: Phospho-p]3-kinase p85-
sterile PH 7.40.01M PBS or diluent of antibody the antibody is stable for
alpha/gamma (Iyr467/199) pab at
100
1/300 dilution
at least two weeks at 2-4.C


ALIASES

Phosphatidylinositol 3-kinase regulatory subunit alpha, Pl3-kinase
regulatory subunit alpha, PI3K regulatory subunit alpha, Ptdins-3-kinase
regulatory subunit alpha, Phosphatidylinositol 3-kinase 85 kda
Goat Anti-rabbit IGG-HRP, 5% skim
milk conjugated at 1/10000 dilution
regulatory subunit alpha, P13-kinase subunit p85-alpha, Ptdlns-3-kinas
regulatory subunit p85-alpha, PIK3R1, GRB 1


REACTIVITY

Human, Mouse, Rat, Dog, Pig, Cow, Horse


ISOTYPE

PREDICTED MOLECULAR WEIGHT

54 kda


Dilution

 

Western blotting
1:500-1:2000
ELISA
1:500-1:1000
HC-P/IHC-F
1:400-1:800
1:100-1:500



Specificity

PI3 Kinase p85 alpha Antibody detects endogenous levels of total PI3 Kinase p85 alpha

Immunogen

A synthesized peptide derived from human PI3 Kinase p85 alpha

Storage Condition and Buffer

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Storage Condition

Store at -20°C long term.

T40115

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