13162017139（微信同号）
13162017139（微信同号）
Ravtansine (DM4) is a maytansinoid, a chemical derivative of maytansine being investigated as the cytotoxic payload of a number of antibody-drug conjugates (ADCs). Microtubules are dynamic cytoskeletal polymers that switch stochastically between states of growing and shortening, called “dynamic instability”. They function in the precise segregation of chromosomes during cell division, transport of cellular cargos, and positioning and movement of intracellular organelles. Inhibition of microtubule function leads to cell cycle arrest and cell death. Microtubule-targeted drugs including the Vinca alkaloids, taxanes, and epothilones suppress the dynamic instability of microtubules, induce mitotic arrest, inhibit cell proliferation and induce apoptosis. The anticancer properties of maytansinoids have been attributed to their ability to disrupt microtubule function. The maytansinoid emtansine (DM1), for example, binds at the ends of microtubules and thereby suppress their dynamic instability. It is synthesized in order to link maytansinoids to antibodies via disulfide bonds. Maytansinoids inhibit tubulin polymerization and microtubule assembly and enhance microtubule destabilization, so there is potent suppression of microtubule dynamics resulting in a mitotic block and subsequent apoptotic cell death. DM4 can be used in the preparation of antibody drug conjugate. Although S-methyl DM1 and S-methyl DM4 inhibited microtubule assembly more weakly than maytansine, they suppressed dynamic instability more strongly than maytansine. Like vinblastine, the maytansinoids potently suppress microtubule dynamic instability by binding to a small number of high affinity sites, most likely at microtubule ends. Thus, the maytansine derivatives that result from cellular metabolism of the antibody conjugates are themselves potent microtubule poisons, interacting with microtubules as effectively as or more effectively than the parent molecule.
Rabbit
Recombinant Rabbit monoclonal Antibody
1 mg/mL.
ELISA
 | Direct ELISA analysis of DM4 was performed by coating wells of a 96-well plate with 50 μl per well of DM4-BSA diluted in carbonate/bicarbonate buffer, at a concentration of 1 μg/mL overnight at 4℃. Wells of the plate were washed, blocked with StartingBlock blocking buffer, and incubated with 50 μl per well of a mouse IL-2 monoclonal antibody starting at a concentration of 10 μg/mL and serially diluting it to a concentration of 0.169 ng/mL for 2 hours at room temperature. The plate was washed and incubated with 50 μl per well of an HRP-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1:15,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 30 minutes at 37℃. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Note: Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.
注意：抗体应用为IHC的，抗体只适合于石蜡切片的组化染色或者荧光染色。
抗体应用为IF/ICC的，抗体适合于冰冻切片的组化染色或者荧光染色，以及细胞水平的化学染色和荧光染色。
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邮箱：market@ab-mart.com
应聘职位：hr@ab-mart.com
订购专线：4006-123-828
销售电话：13162017139（微信同号）
技术支持：13162477137（微信同号）
总机：021-34695901
经销商：QQ 402772198
南方经销商负责：手机13122837132(微信同号)
北方及西南经销商负责：手机13122150513(微信同号)
微信客服
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