重组蛋白-Recombinant Human Novel Coronavirus Spike glycoprotein(S),partial (Active)

Datasheet

Purity

Greater than 90% as determined by SDS-PAGE.

Endotoxin

Not test.

Bioactivity

①Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 2 μg/ml can bind SARS-CoV-2-S Antibody (CSB-RA33245A1GMY), the EC50 of SARS-CoV-2-S1-RBD protein is 27.96 - 33.35 ng/ml.
②Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 2 μg/ml can bind SARS-CoV-2-S Antibody (CSB-RA33245A0GMY), the EC50 of SARS-CoV-2-S1-RBD protein is 13.96 -16.62 ng/ml.
③Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 5 μg/ml can bind human ACE2 (CSB-MP866317HU), the EC50 is 2.785-9.139 ng/ml.
④SARS-CoV-2 Spike protein RBD his/myc tag (CSB-MP3324GMY1b1) captured on COOH chip can bind Human ACE2 protein Fc tag (CSB-MP866317HU) with an affinity constant of 13.8 nM as detected by LSPR Assay.
⑤Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 2 μg/ml can bind Biotinylated human ACE2 (CSB-MP866317HU-B), the EC50 is 4.599-8.322 ng/ml.

Gene Name

Uniprot

P0DTC2

Application

Organism

Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2)

Host

Mammalian cell

Mol Weight

30.1 kDa

Expression Region

319-541aa

Tag

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Formulation

Lyophilized powder

Buffer

Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Storage

Notes

Partial

Lead Time

3-7 business days

Image

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 2 μg/ml can bind SARS-CoV-2-S Antibody (CSB-RA33245A1GMY), the EC50 of SARS-CoV-2-S1-RBD protein is 27.96 - 33.35 ng/ml.

Activity
Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 2 μg/ml can bind SARS-CoV-2-S Antibody (CSB-RA33245A0GMY), the EC50 of SARS-CoV-2-S1-RBD protein is 13.96 -16.62 ng/ml.

Activity
Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 5 μg/ml can bind human ACE2 (CSB-MP866317HU), the EC50 is 2.785-9.139 ng/ml.

Activity
SARS-CoV-2 Spike protein RBD his/myc tag (CSB-MP3324GMY1b1) captured on COOH chip can bind Human ACE2 protein Fc tag (CSB-MP866317HU) with an affinity constant of 13.8 nM as detected by LSPR Assay.

Activity
Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 2 μg/ml can bind Biotinylated human ACE2 (CSB-MP866317HU-B), the EC50 is 4.599-8.322 ng/ml

MHY24G2

货号 规格 价格(¥)
MHY24G2H 1mg
MHY24G2L 100ug
MHY24G2M 20ug

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Note:  Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.

注意:抗体应用为IHC的,抗体只适合于石蜡切片的组化染色或者荧光染色。

抗体应用为IF/ICC的,抗体适合于冰冻切片的组化染色或者荧光染色,以及细胞水平的化学染色和荧光染色。

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微信客服

邮箱:market@ab-mart.com

应聘职位:hr@ab-mart.com

订购专线:4006-123-828

销售电话:13162017139(微信同号)

技术支持:13162477137(微信同号)

总机:021-34695901

经销商:QQ 402772198
南方经销商负责:手机13122837132(微信同号)          
北方及西南经销商负责:手机13122150513(微信同号)

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