细胞因子-Recombinant Noggin, Human, AF

Datasheet

Synonym/Alternative name(s)

NOG, Noggin, SYM1, symphalangism 1 (proximal), synostoses (multiple) syndrome 1, SYNS1, SYNS1A

Activity

Measure by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 cells.
The ED50 for this effect is <0.05 μg/mL in the presence of 50 ng/mL of recombinant human BMP-4.

Protein Description

Noggin is a bioactive protein intended for use in cell culture applications. Noggin protein which binds to ligands of the TGF-β family and regulates their activity by inhibiting their access to signaling receptors. Noggin was originally identified as a BMP-4 antagonist whose action is critical for proper formation of the head and other dorsal structures.

Protein Accession

Q13253.1

Gene ID

9241

Application

Expression Region

MQHYLHIRPAPSDNLPLVDLIEHPDPIFDPKEKDLNETLLRSLLGGHYDPGFMATSPPEDRPGGGGGAAGGAEDLAELDQLLRQRPSGAMPSEIKGLEFSEGLAQGKKQRLSKKLRRKLQMWLWSQTFCPVLYAWNDLGSRFWPRYVKVGSCFSKRSCSVPEGMVCKPSKSVHLTVLRWRCQRRGGQRCGWIPIQYPIISECKCSC with polyhistidine tag at the C-terminus.

Tag

His-tag?at?the?C-terminus

Species

Human

Reactivity

Human

Expression Host

Escherichia coli

Source

E. coli

Purification

>98% as determined by SDS-PAGE. Ni-NTA chromatography

Endotoxin

<0.1 EU per 1 μg of the protein by the LAL method.

Calculated Molecular Weight

24 kDa

Formulation

The protein was lyophilized from a solution containing 0.1% sarkosyl in 1X PBS, pH 8.0.

Reconstitution

It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

Shipping

Blue Ice

Stability and Storage

Lyophilized protein should be stored at -20°C for 1 year.
Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1%
BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months.

Category

Cytokines

Application

Cell culture, Elisa

Image

SDS- PAGE analysis of recombinant human Noggin

RMCH172

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RMCH172S 5ug
RMCH172M 20ug
RMCH172L 100ug
RMCH172H 500ug

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Note:  Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.

注意:抗体应用为IHC的,抗体只适合于石蜡切片的组化染色或者荧光染色。

抗体应用为IF/ICC的,抗体适合于冰冻切片的组化染色或者荧光染色,以及细胞水平的化学染色和荧光染色。

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