Microtubules are cylindrical tubes of 20-25 nm in diameter. They are composed of protofilaments which are in turn composed of alpha- and beta- tubulin polymers. Each microtubule is polarized, at one end alpha-subunits are exposed (-) and at the other beta-subunits are exposed (+). Microtubules act as a scaffold to determine cell shape, and provide a backbone for cell organelles and vesicles to move on, a process that requires motor proteins. The major microtubule motor proteins are kinesin, which generally moves towards the (+) end of the microtubule, and dynein, which generally moves towards the (-) end. Microtubules also form the spindle fibers for separating chromosomes during mitosis.

TUBB1, TUBB5, tubulin beta, TUBB

50kDa

This Abmart monoclonal antibody is produced by immunizing mice with a recombinant polypeptide (Abmart SEAL mAb technology) corresponding to surface multi-epitopes of beta-tubulin.

Mouse IgG

Beta tubulin monoclonal antibody detects endogenous levels of beta tubulin protein.

H, Mk, M, ChHm, Dg, R

Western blotting        1:1000-1:2000

Use an anti-MOUSE secondary antibody.

Store at -20°C, stable for one year from the date of shipment.

AB_2916070

1.       Volz A, Weiss E, Hum Genet. 1994 Jan;93(1):42-6.

2.       Hall JL, Dudley L, Mol Cell Biol. 1983 May;3(5):854-62

#M21001 Goat anti-mouse IgG-HRP