Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties. The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion.
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This Abmart monoclonal antibody is produced by immunizing animals with a synthetic DYKDDDDK peptide (KLH-coupled).
DYKDDDDK Tag Antibody detects exogenously expressed DYKDDDDK proteins in cells.
Store at -20°C. Stable for one year from the date of shipment.
All
Mouse IgG2b
Use an anti-Mouse?secondary antibody to detect the 3B9 antibody.
Western blotting 1:5000
Immunofluorescence 1:1000
Immunoprecipitation 1:100
ELISA 1:2000
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#M20023 β-Tubulin Antibody(HRP Conjugated)
#M20024 GAPDH (3B3)Antibody(HRP Conjugated)
#M20025 Anti-GST-Tag Antibody(HRPConjugated)
#M20026 Anti-DYKDDDDK-Tag Antibody(HRPConjugated)
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