标签Tag-MBP-Tag(4M14) Mouse mAb

Datasheet

Background

All Names: MalE; malJ; Maltodextrin binding protein; Maltose ABC

transporter periplasmic protein; Maltose binding periplasmic protein;

MMBP; Periplasmic maltose binding protein;

Antibodies derived from MBP fusion proteins a re  useful for checking

protein expression both in plaques and on Western blots as well as for

immunoaffinity purification of proteins expressed in E. Coli.

Source

This Abmart monoclonal antibody is produced by immunizing

animals with full-length MBP protein.

Specificity

MBP-Tag (4M14) Mouse mAb detects MBP and MBP fusion proteins.

Storage

Store at -20°C. Stable for one year from the date of shipment.

Application

Reactivity

Important

Use an anti-MOUSE secondary antibody to detect the 4M14 antibody.

Recommanded Dilutions

Western blotting 1:2000—1:5000

Immunofluorescence 1:2000

Immunoprecipitation 1:100

ELISA 1:2000

*Western blots, incubate membrane with diluted antibody in 5%

w/v nonfat drmilk, 1x TBS. 0.05% Tween-20 at 4 ° C with ge ” tie

shaking, overnight.

Companion products

#M20001 His-Tag (2A8) Mouse mAb

#M20002 Myc-Tag (19C2) Mouse mAb

#M20003 HA-Tag (26D11) Mouse mAb

#M20004 GFP-Tag (7G9) Mouse mAb

#M20008 DYDDDDDK-Tag (3B9) Mouse mAb (Binds to same

epitope as Sigma’s Anti-FLAG M2 Antibody)

#M20012 Anti-Myc-Tag Mouse mAb (Agarose Conjugated)

#M20013 Anti-HA-Tag Mouse mAb (Agarose Conjugated)

#M20018 Anti-DYKDDDDK-Tag Mouse mAb (Agarose Conjugated)

(Binds to same epitope as Sigma’s Anti-FLAG M2 Antibody)

M20051

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