The catenins, α,? and y, are proteins which bind to the highly conserved, intracellular cytoplasmic tail of E-cadherin. Together, the catenin/cadherin complexes play an important role mediating cellular adhesion. α-catenin was initially  described as an E-cadherin
associated protein, and since has been shown to associate with other members of the cadherin family, such as N- cadherin and P-cadherin. ?-catenin associates with the cytoplasmic portion of  E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. ?-catenin has also been found in complexes with the tumor suppressor  protein  APC. y-catenin, also known as plakoglobin, is a protein that binds with α-catenin and N-cadherin. It has been shown that the
transmembrane phosphatase PTPμ associates with catenin/cadherin complexes and may regulate complex signaling.

P35222 

This Abmart monoclonal antibody is produced by immunizing mice with a synthetic  peptide (KLH-coupled) corresponding to carboxy-terminal  residues of human ?-catenin.

?-Catenin (44C6) Mouse  mAb detects  endogenous  levels of total  ?-catenin protein.

Store at -20°C, stable for one year from the date of shipment.

92kDa

Human, Mouse, Rat

1. Mouse IgG1
   

1. 1. Centrifuge to settle the lyophilized antibody to the bottom of the vial.
   Reconstitute using 100 μl Antibody Dilution Buffer.
   

2. 2. Use an anti-MOUSE secondary antibody to detect the 44C6 antibody.

Western blotting                    1:1000
Immunofluorescence           1:200
Immunohistochemistry        1:200

*For Western blots, incubate membrane with diluted antibody in 5% w/v non-fat dry milk,  1× TBS, 0.05% Tween-20  at 4°C with gentle shaking, overnight.

#M21001 Goat Anti-Mouse  IgG-HRP