Rat

IgA

Monoclonal

Protoplasts prepared from suspension-cultured cells

JIM7

Hybridoma supernatant

Carrot (Daucus carota)

JIM7 binds to a relatively heavily methylesterified epitope found in cell walls of diverse species. The epitope structure shown is the most efficient competitor identified. Other competitors that are almost equally effective are: GalA1-gt;4GalA1-gt;4MeGalA1-gt;4MeGalA1-gt;4MeGalA1-gt;4GalA and MeGalA1-gt;4GalA1-gt;4MeGalA1-gt;4GalA1-gt;4MeGalA1-gt;4GalA.

Homogalacturonan

(partially methyl-esterified)

GalA1-gt;4MeGalA1-gt;4MeGalA1-gt;4MeGalA1-gt;4MeGalA1-gt;4GalA

PlantProbes

Store at 4℃. Stable for 12 months from date of receipt

Arabidopsis inflorescence stem expansion glycoprofiling

J.P. Knox, P.J. Linstead, J. King, C. Cooper, and K. Roberts. (1990) Pectin esterification is spatially regulated both within cell walls and between developing tissues of root apices. Planta 181:512-521.

W.G.T. Willats, G. Limberg, H.C. Buchholt, G.-J. Van Alebeek, J. Benen, T.M.I.E. Christensen, J. Visser, A. Voragen, J.D. Mikkelsen, and J.P. Knox. (2000) Analysis of pectic epitopes recognised by hybridoma and phage display monoclonal antibodies using defined oligosaccharides, polysaccharides, and enzymatic degradation. Carbohydr.Res. 327:309-320, 2000.

M.H. Clausen, W.G.T. Willats, and J.P. Knox. (2003) Synthetic methyl hexagalacturonate hapten inhibitors of anti-homogalacturonan monoclonal antibodies LM7, JIM5 and JIM7. Carbohydr.Res. 338:1797-1800, 2003.

http://glycomics.ccrc.uga.edu/wall2/jsp/abdetails.jsp?abnumber=14amp;abname=LM20