Rat

IgG2a

Monoclonal

Protoplast prepared from suspension-cultured cells

JIM5

Hybridoma supernatant

Carrot (Daucus carota)

JIM5 binds to a sparsely methylated homogalacturonan epitope found in the walls of diverse plant species. The epitope structure listed above is the most effective structure identified to date. JIM5 also binds to unesterified homogalacturonan. JIM5 can also be obtained from Plant Probes (http://www.plantprobes.co.uk/).

Homogalacturonan

(partially methyl-esterified)

Me(alpha)GalA1-gt;4(alpha)GalA1-gt;4(alpha)GalA1-gt;4(alpha)GalA1-gt;4(alpha)GalA1-gt;4(alpha)MeGalA

CarboSource

Store at 4℃. Stable for 12 months from date of receipt

Arabidopsis inflorescence stem expansion glycoprofiling

K.A. VandenBosch, D.J. Bradley, J.P. Knox, S. Perotto, G.W. Butcher, and N.J. Brewin. (1989) Common components of the infection thread matrix and the intercellular space identified by immunocytochemical analysis of pea nodules and uninfected roots. EMBO J.8:335-342.

J.P. Knox, P.J. Linstead, J. King, C. Cooper, and K. Roberts. (1990) Pectin esterification is spatially regulated both within cell walls and between developing tissues of root apices. Planta181:512-521.

W.G.T. Willats, G. Limberg, H.C. Buchholt, G.-J. Van Alebeek, J. Benen, T. M.I.E. Christensen, J. Visser, A. Voragen, J.D. Mikkelsen, and J.P. Knox. (2000) Analysis of pectic epitopes recognised by hybridoma and phage display monoclonal antibodies using defined oligosaccharides, polysaccharides, and enzymatic degradation. Carbohydr.Res.327:309-320.

M.H. Clausen, W.G.T. Willats, and J.P. Knox. (2003) Synthetic methyl hexagalacturonate hapten inhibitors of anti-homogalacturonan monoclonal antibodies LM7, JIM5 and JIM7. Carbohydr.Res.338:1797-1800.

http://glycomics.ccrc.uga.edu/wall2/jsp/abdetails.jsp?abnumber=14amp;abname=LM20