Produces green light with a wavelength of 562 nm. Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.

Luciferin 4-monooxygenase (Luciferase) (EC 1.13.12.7)

Firefly

WB,ICC/IF

WB 1:500~1:2000 ICC/IF 1:50~1:200

55kDa

p08659

https://www.uniprot.org/uniprotkb/p08659

A synthesized peptide derived from Firefly Luciferase

Rabbit

Monoclonal

Rabbit IgG

Firefly Luciferase Antibody detects endogenous levels of total Firefly Luciferase

Luminescence #bioluminescence

Affinity-chromatography

PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol

Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

AB_2936909