All Names: MalE; malJ; Maltodextrin binding protein; Maltose ABC

transporter periplasmic protein; Maltose binding periplasmic protein;

MMBP; Periplasmic maltose binding protein;

Antibodies derived from MBP fusion proteins a re useful for checking

protein expression both in plaques and on Western blots as well as for

immunoaffinity purification of proteins expressed in E. Coli.

All

WB

Western blotting 1:2000—1:5000 Immunofluorescence 1:2000 Immunoprecipitation 1:100 ELISA 1:2000* Western blots, incubate membrane with diluted antibody in 5%w/v nonfat drmilk, 1x TBS. 0.05% Tween-20 at 4 ° C with ge ” tieshaking, overnight.

This Abmart monoclonal antibody is produced by immunizinganimals with full-length MBP protein.

Mouse

Monoclonal

Mouse IgG

MBP-Tag (4M14) Mouse mAb detects MBP and MBP fusion proteins.

PBS, pH 7.4, 40% glycerol, and 0.01% sodium azide.

Store at -20°C. Stable for one year from the date of shipment.

#M20001 His-Tag (2A8) Mouse mAb

#M20002 Myc-Tag (19C2) Mouse mAb

#M20003 HA-Tag (26D11) Mouse mAb

#M20004 GFP-Tag (7G9) Mouse mAb

#M20008 DYDDDDDK-Tag (3B9) Mouse mAb (Binds to same

epitope as Sigma's Anti-FLAG M2 Antibody)

#M20012 Anti-Myc-Tag Mouse mAb (Agarose Conjugated)

#M20013 Anti-HA-Tag Mouse mAb (Agarose Conjugated)

#M20018 Anti-DYKDDDDK-Tag Mouse mAb (Agarose Conjugated)

(Binds to same epitope as Sigma's Anti-FLAG M2 Antibody)

Use an anti-MOUSE secondary antibody to detect the 4M14 antibody.

AB_2936269