Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (EC 1.2.1.12) (Peptidyl-cysteine S-nitrosylase GAPDH) (EC 2.6.99.-) GAPDH GAPD CDABP0047 OK/SW-cl.12

Human, Mouse, Rat, Pig, Bovine, Goat, Monkey, Chicken

Horse,Sheep,Rabbit,Dog,Xenopus

WB,IHC,IF/ICC,ELISA

WB,IHC,IF/ICC, ELISA Recommended dilution WB 1:500-1:2000 IHC 1:50-1:500 IF/ICC 1:50-1:500 ELISA 1：2000

37kD; 36kDa(Calculated).

GAPDH GAPD CDABP0047 OK/SW-cl.12

P04406

https://www.uniprot.org/uniprotkb/p04406

Rabbit

Polyclonal

Rabbit IgG

GAPDH antibody detects endogenous levels of total GAPDH.

Glucose metabolism #Glycolysis #Cytolysis #defense response to fungus #killing of cells of another organism #negative regulation of translation

PBS,pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol

Store at -20 °C. Stable for 12 months from date of receipt.