Immunofluorescence staining of Hela cells with MH15034 at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunofluorescence staining of MCF-7 cells with MH15034 at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunofluorescence staining of SW620 cells with MH15034 at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

Overlay histogram showing MCF-7 cells stained with MH15034 (red line) at 1:100. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.

Western Blot
Positive WB detected in: Caco205 whole cell lysate
All lanes: CDH1 antibody at 1:1000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 98, 91 kDa
Observed band size: 110, 120 kDa