Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly. Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface. Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility. Binds DNA, with a preference for supercoiled DNA and AT-rich DNA. Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization. It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable).

Proliferation marker protein Ki-67 (Antigen identified by monoclonal antibody Ki-67) (Antigen KI-67) (Antigen Ki67)MKI67

Human, Mouse, Rat

WB,IHC,IF/ICC, FCM

Recommended dilution WB 1:500-1:2000 IHC 1:50-1:500 IF/ICC 1:50-1:500 FCM 1:50-1:200

358kD; 359kDa(Calculated).

MKI67

P46013

https://www.uniprot.org/uniprotkb/p46013

Rabbit

Monoclonal

Rabbit IgG

Ki67 Antibody detects endogenous levels of total Ki67.

Nucleus localization

Cell cycle

PBS with 0.02% sodium azide, 100ug/ml BSA and 50% glycerol.

Store at -20 °C. Stable for 12 months from date of receipt.