Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties. The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems.

All

WB,IF,IHC,IP/ChIP,ELISA

Western blotting 1:5000 Immunofluorescence 1:2000 Immunoprecipitation 1:200 ELISA 1:2000

This Abmart monoclonal antibody is produced by immunizing animals with a synthetic peptide (KLH-coupled) corresponding to residues 410-419 of human c-Myc (EQKLISEEDL).

Mouse

Monoclonal

Mouse IgG1

Myc-Tag (19C2) Mouse mAb detects over-expressed or recombinant proteins containing the Myc epitope tag.

PBS, pH 7.4, 40% glycerol, and 0.01% sodium azide.

Store at -20°C. Stable for one year from the date of shipment.

#M20001 His-Tag (2A8) Mouse mAb
#M20003 HA-Tag (26D11) Mouse mAb
#M20004 GFP-Tag (7G9) Mouse mAb
#M20007 GST-Tag (12G8) Mouse mAb
#M20008 DYDDDDDK-Tag (3B9) Mouse mAb (Binds to same epitope as Sigma's Anti-FLAG M2 Antibody)
#M20012 Anti-Myc-Tag Mouse mAb (Agarose Conjugated)
#M20013 Anti-HA-Tag Mouse mAb (Agarose Conjugated)
#M20018 Anti-DYKDDDDK-Tag Mouse mAb (Agarose Conjugated) (Binds to same epitope as Sigma's Anti-FLAG M2 Antibody)

AB_2861172

Munro, S. and Pelham, H.R. 1984. Use of peptide tagging to detect proteins expressed from cloned genes: deletion mapping functional domains of Drosophila hsp 70. EMBO J. 3, 3087-3093.