Plasmid vectors for the expression of coding regions of eukaryotic genes in E.coli are in common usage; such expression vectors often encode hybrid fusion proteins containing part prokaryotic and part eukaryotic specified proteins. For instance, the pGEX.3X expression vector developed by Smith and Johnson allows for synthesis of fusion proteins between glutathionine-S-transferase
(GST) and proteins encoded by inserted cDNA sequences. Antibodies derived from these GST fusion proteins are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in E. Coli.

All

WB

Western blotting 1:5000 Immunofluorescence 1:2000 Immunoprecipitation 1:100 ELISA 1:2000

This Abmart monoclonal antibody is produced by immunizing animals with GST.

Mouse

Monoclonal

Mouse IgG1

GST-Tag (12G8) Mouse mAb detects GST and GST fusion proteins.

PBS, pH 7.4, 40% glycerol, and 0.01% sodium azide.

Store at -20°C. Stable for one year from the date of shipment.

#M20001 His-Tag (2A8) Mouse mAb
#M20002 Myc-Tag (19C2) Mouse mAb
#M20003 HA-Tag (26D11) Mouse mAb
#M20004 GFP-Tag (7G9) Mouse mAb
#M20008 DYDDDDDK-Tag (3B9) Mouse mAb (Binds to same epitope as Sigma's Anti-FLAG M2 Antibody)
#M20012 Anti-Myc-Tag Mouse mAb (Agarose Conjugated)
#M20013 Anti-HA-Tag Mouse mAb (Agarose Conjugated)
#M20018 Anti-DYKDDDDK-Tag Mouse mAb (Agarose Conjugated) (Binds to same epitope as Sigma's Anti-FLAG M2 Antibody)

AB_2864360

1.Maniatis, T., Fritsch, E.F. and Sambrook, J. 1982. Molecular Cloning- A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
2. Smith, D.B. and Johnson, K.S. 1988. Single-step purification of polypeptides expressed in Escherichia coli as fusions with Glutathione S-transferase. Gene 67: 31-40.
3. Crabb, B.S. and Studdert, M.J. 1995. Expression of small regions of equine herpesvirus 1 glycoprotein C in Escherichia coli. Vet. Microbiol. 46: 181-191.
4. Soler, D., Nomizu, T., Brown, W.E., Shibata, Y. and Auld, D.S. 1995. Matrilysin: expression, purification, and characterization. J. Protein Chem.14: 511-520.