Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties. The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion.

All

WB,IF,IHC,IP/ChIP

Western blotting 1:5000 Immunofluorescence 1:1000 Immunoprecipitation 1:100 ELISA 1:2000

This Abmart monoclonal antibody is produced by immunizing animals with a synthetic DYKDDDDK peptide (KLH-coupled).

Mouse

Monoclonal

Mouse IgG2b

DYKDDDDK Tag Antibody detects exogenously expressed DYKDDDDK proteins in cells.

PBS with 0.02% sodium azide, 100ug/ml BSA and 50% glycerol.

Store at -20°C. Stable for one year from the date of shipment.

#M20001 His-Tag (2A8) Mouse mAb
#M20002 Myc-Tag (19C2) Mouse mAb
#M20003 HA-Tag (26D11) Mouse mAb
#M20004 GFP-Tag (7G9) Mouse mAb
#M20007 GST-Tag (12G8) Mouse mAb
#M20012 Anti-Myc-Tag Mouse mAb (Agarose Conjugated)
#M20013 Anti-HA-Tag Mouse mAb (Agarose Conjugated)
#M20018 Anti-DYKDDDDK-Tag Mouse mAb (Agarose Conjugated) (Binds to same epitope as Sigma's Anti-FLAG M2 Antibody)

AB_2713960

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2. RGF1 INSENSITIVE 1 to 5, a group of LRR receptor-like kinases, are essential for the perception of root meristem growth factor 1 in Arabidopsis thaliana. Cell Res. 2016 Jun;26(6):686-98

3. Degradation of MONOCULM 1 by APC/C(TAD1) regulates rice tillering. Nat Commun. 2012 Mar 20;3:750.

4. Loss of RIG-I leads to a functional replacement with MDA5 in the Chinese tree shrew. Proc Natl Acad Sci U S A. 2016 Sep 27;113(39):10950-5

5. SINAT E3 Ligases Control the Light-Mediated Stability of the Brassinosteroid-Activated Transcription Factor BES1 inArabidopsis. Dev Cell. 2017 Apr 10;41(1):47-58.e4

6. The E3 Ligase DROUGHT HYPERSENSITIVE Negatively Regulates Cuticular Wax Biosynthesis by Promoting the Degradation of Transcription Factor ROC4 in Rice. Plant Cell. 2017 Dec 13. pii: tpc.00823.2017.

7. Interaction of amyotrophic lateral sclerosis/frontotemporal lobar degeneration-associated fused-in-sarcoma with proteins involved in metabolic and protein degradation pathways. Neurobiol Aging. 2015 Jan;36(1):527-35.

8. A novel P53/POMC/Gαs/SASH1 autoregulatory feedback loop activates mutated SASH1 to cause pathologic hyperpigmentation. J Cell Mol Med. 2017 Apr;21(4):802-815.

9. Proteomics strategy to identify substrates of LNX, a PDZ domain-containing E3 ubiquitin ligase.J Proteome Res. 2012 Oct 5;11(10):4847-62.

10. HSP40 interacts with pyruvate kinase M2 and regulates glycolysis and cell proliferation in tumor cells. PLoS One. 2014 Mar 21;9(3):e92949.

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