The green fluorescent protein (GFP) was originally identified as a protein involved in the bioluminescence of the jellyfish Aequorea victoria. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors, making GFP a useful tool for monitoring gene expression and protein localization in vivo. Several GFP mutants have been developed, including EGFP, which fluoresce more intensely than the wildtype GFP and have shifted excitation maxima, making them useful for FACS and fluorescence microscopy as well as double-labeling applications. GFP is widely used in expression vectors as a fusion protein tag, allowing expression and monitoring of heterologous proteins fused to GFP.

All

WB,IF,IP,ELISA

Western blotting 1:5000 Immunofluorescence 1:500 Immunoprecipitation 1:50 ELISA 1:2000

This Abmart monoclonal antibody is produced by immunizing animals with full length recombinant GFP.

Mouse

Monoclonal

Mouse IgG1

GFP-Tag (7G9) Mouse mAb detects GFP and GFP fusion proteins.

PBS, pH 7.4, 40% glycerol, and 0.01% sodium azide.

Store at -20°C. Stable for one year from the date of shipment.

#M20001 His-Tag (2A8) Mouse mAb
#M20002 Myc-Tag (19C2) Mouse mAb
#M20003 HA-Tag (26D11) Mouse mAb
#M20007 GST-Tag (12G8) Mouse mAb
#M20008 DYDDDDDK-Tag (3B9) Mouse mAb (Binds to same epitope as Sigma's Anti-FLAG M2 Antibody)
#M20012 Anti-Myc-Tag Mouse mAb (Agarose Conjugated)
#M20013 Anti-HA-Tag Mouse mAb (Agarose Conjugated)
#M20018 Anti-DYKDDDDK-Tag Mouse mAb (Agarose Conjugated) (Binds to same epitope as Sigma's Anti-FLAG M2 Antibody)

AB_2619674

1. Prasher, D.C., et al. 1992. Primary structure of the Aequorea victoria green fluorescent protein. Gene 111: 229-233.
2. Chalfie, M., et al. 1994. Green fluorescent protein as a marker for gene expression. Science 263: 802-805.