Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. Though differentially expressed from tissue to tissue, GAPDH is thought to be a constitutively expressed housekeeping protein. For this reason, GAPDH mRNA and protein levels are often measured as controls in experiments quantifying specific changes in expression of other targets. Recent work has elucidated roles for GAPDH in apoptosis, gene expression and nuclear transport. GAPDH may also play a role in neurodegenerative pathologies such as Huntington and Alzheimer's diseases.

Human, Monkey,Bacillussubtilis

WB

Western blotting 1:1000

37 kDa

This Abmart polyclonal antibody is produced by immunizing animals with a synthetic peptide (KLH-coupled) corresponding to carboxy-terminal residues of human GAPDH.

Rabbit

Polyclonal

Rabbit IgG

GAPDH Antibody detects endogenous levels of total GAPDH protein.

PBS, pH 7.4, 40% glycerol, and 0.01% sodium azide.

Store the product at -20°C. Stable for one year from the date of shipment.

#M20005 b-Tubulin (10B1) Mouse mAb

#M20006 GAPDH (3B3) Mouse mAb

#P30002 b-Actin Antibody

#M21002 Goat Anti-Rabbit IgG-HRP

Use an anti-RABBIT secondary antibody to detect the antibody.

AB_2936506

1. Barber, R.D. et al. 2005. GAPDH as a housekeeping gene: analysis of GAPDH mRNA expression in a panel of 72 human tissues. Physiol. Genomics 21,

389-95. 2. Hara, M.R. and Snyder, S.H. 2006. Nitric oxide-GAPDH-Siah: a novel cell death cascade. Cell Mol. Neurobiol. 26, 527-38.

3. Zheng, L. et al. 2003. S phase activation of the histone H2B promoter by OCA-S, a coactivator complex that contains GAPDH as a key component. Cell 114, 255-66.

4. Bae, B.I. et al. 2006. Mutant huntingtin: nuclear translocation and cytotoxicity mediated by GAPDH. Proc. Natl. Acad. Sci. USA 103, 3405-9.

5. Wang, Q. et al. 2005. Proteomic analysis of neurofibrillary tangles in Alzheimer disease identifies GAPDH as a detergent-insoluble paired helical filament tau binding protein. FASEB J. 19, 869-71.