二抗-通用型避免抗体重链+轻链二抗

Datasheet

Description

Background

50kD or 25kD protein detection on Western blots after Immunoprecipitation is often suffered from heavy or light chain blotting contamination. AbSmart products (M21008) solve these problems through less background noise, and enhanced accuracy.

Alternative Name

Reactivity

Mouse,Rat,Rabbit,Guinea pig,Human,Monkey,Pig,Cow,Horse, Dog,Goat,Sheep,Llama. Only for use to IgG, NOT for ues to IgA, IgM, IgD, IgE, IgY ect.

Specificity

This secondary antibody for IP Detection Reagent (HRP) preferentially detects the non-reduced form over the reduced, SDS-denatured forms. IP sample should be completely reduced/denatured before loaded onto a western blot.

When used for IP, if the primary antibody is rabbit IgG, this secondary antibody will slightly recognize the heavy chain of denatured rabbit IgG, thus failing to achieve the goal of avoiding antibody heavy chains.

Application Image

Application

WB,IHC,IP,ELISA

Recommended Dilution

ELISA 1:1000 - 1 :5000

WB 1:1000 - 1:5000

IHC 1:500 - 1:2000
IP 1:2000 - 1 :5000

Mol Weight

Immunogen

Clonality

Monoclonal

Isotype

Purification

Conjugation

HRP

Excitation/Emission Max

Buffer

PBS,pH 7.4,40% Glycerol,and 0.01% sodium azide.

Concentration

注意事项

如用于IP,对于兔IgG的重链会有轻微识别,对于兔IgG重链的去除建议选用#M21009(鼠+兔通用避免抗体重链+轻链二抗)

RRID

Companion Products

Storage

Store at -20°C. Stablefor one yearfrom the date of shipment.

M21008

货号 规格 价格(¥)
M21008S 100μl
M21008M 500μl
M21008L 1000μl

以上产品均有现货供应

订购咨询 orders@ab-mart.com

订货热线 4006-123-828

技术支持15618194176

经销商名录:点击查看


Note:  Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.

注意:抗体应用为IHC的,抗体只适合于石蜡切片的组化染色或者荧光染色。

抗体应用为IF/ICC的,抗体适合于冰冻切片的组化染色或者荧光染色,以及细胞水平的化学染色和荧光染色。

艾比玛特医药科技(上海)有限公司
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邮箱:market@ab-mart.com

应聘职位:hr@ab-mart.com

订购专线:4006-123-828

销售电话:13162017139(微信同号)

技术支持:15618194176(微信同号)

南方经销商负责:手机13122837132(微信同号)          
北方及西南经销商负责:手机13122150513(微信同号)

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微信客服

邮箱:market@ab-mart.com

应聘职位:hr@ab-mart.com

订购专线:4006-123-828

销售电话:13162017139(微信同号)

技术支持:15618194176(微信同号)

总机:021-34695901

南方经销商负责:手机13122837132(微信同号)          
北方及西南经销商负责:手机13122150513(微信同号)

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微信客服

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