50kD or 25kD protein detection on Western blots after Immunoprecipitation is often suffered from heavy or light chain blotting contamination. AbSmart products (M21008) solve these problems through less background noise, and enhanced accuracy.
Mouse,Rat,Rabbit,Guinea pig,Human,Monkey,Pig,Cow,Horse, Dog,Goat,Sheep,Llama. Only for use to IgG, NOT for ues to IgA, IgM, IgD, IgE, IgY ect.
This secondary antibody for IP Detection Reagent (HRP) preferentially detects the non-reduced form over the reduced, SDS-denatured forms. IP sample should be completely reduced/denatured before loaded onto a western blot.
When used for IP, if the primary antibody is rabbit IgG, this secondary antibody will slightly recognize the heavy chain of denatured rabbit IgG, thus failing to achieve the goal of avoiding antibody heavy chains.
WB,IHC,IP,ELISA
ELISA 1:1000 - 1 :5000
WB 1:1000 - 1:5000
IHC 1:500 - 1:2000
IP 1:2000 - 1 :5000
Monoclonal
HRP
PBS,pH 7.4,40% Glycerol,and 0.01% sodium azide.
如用于IP,对于兔IgG的重链会有轻微识别,对于兔IgG重链的去除建议选用#M21009(鼠+兔通用避免抗体重链+轻链二抗)
Store at -20°C. Stablefor one yearfrom the date of shipment.
Note: Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.
注意:抗体应用为IHC的,抗体只适合于石蜡切片的组化染色或者荧光染色。
抗体应用为IF/ICC的,抗体适合于冰冻切片的组化染色或者荧光染色,以及细胞水平的化学染色和荧光染色。
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邮箱:market@ab-mart.com
应聘职位:hr@ab-mart.com
订购专线:4006-123-828
销售电话:13162017139(微信同号)
技术支持:15618194176(微信同号)
总机:021-34695901
南方经销商负责:手机13122837132(微信同号)
北方及西南经销商负责:手机13122150513(微信同号)
微信客服
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