Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties. The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion.

All

WB

WB1:500-1:2000lmmunofluorescence 1 :100-1:1000lmmunoprecipitation 1 :100 ELISA 1 :2000

This antibody is produced by immunizing animals with a synthetic DYKDDDDK peptide (KLH-coupled).

Rabbit

Monoclonal

Rabbit IgG

DYKDDDDK Tag Antibody detects exogenously expressed DYKDDDDK proteins in cells.

PBS, pH 7.4, 40% glycerol, and 0.01% sodium azide.

Store at -20 °C. Stable for 12 months from date of receipt.

AB_2936781