表观产品-小分子抑制剂-Acetyl-Histone H4-K12 Rabbit pAb

Datasheet

BACKGROUND

Histones are basic nuclear proteins that are responsible for the nucleosome structure of  the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones  (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA  is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with  linker DNA between nucleosomes and functions in the compaction of chromatin into  higher order structures. This gene is intronless and encodes a replication-dependent  histone that is a member of the histone H4 family. Transcripts from this gene lack polyA  tails but instead contain a palindromic termination element. This gene is found in the  large histone gene cluster on chromosome 6.


Alternative Names

HIST1H4B;H4/I;H4FI;histone H4;Histone H4;HIST1H4A;HIST2H4A;FO108;H4;H4/ n;H4F2;H4FN;HIST2H4

SOURCE

A synthetic acetylated peptide around K12 of human Histone H4 .

STORAGE

Store at -20 Stable far one year from the date of shipment.  

REACTIVITY

Human, Mouse, Rat, Other (Wide Range)

ISOTYPE

RECOMMENDED ANTIBODY DILIITIONS

Western blotting   1:500-1:2000  

IF/IHC/IP   1:50-1:200 

ChIP   1:20-1:100

APPLICATION

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Western blot analysis of extracts of C2C12 cells, using Acetyl-Histone H4- K12 antibody at 1:1000 dilution.C2C12 cells were treated by TSA (1 uM) at  37 for 18 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25ug per lane.

T914227

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