表观产品-小分子抑制剂-Acetyl-Histone H3-K9 Rabbit pAb

Datasheet

BACKGROUND

Histones are basic nuclear proteins that are responsible for the nucleosome structure  of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp  of DNA wrapped around a histone octamer composed of pairs of each of the four core  histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through  the interaction of a linker histone, H1, with the DNA between the nucleosomes to  form higher order chromatin structures. This gene is intronless and encodes a  replicationdependent histone that is a member of the histone H3 family. Transcripts  from this gene lack polyA tails; instead, they contain a palindromic termination  element. This gene is located separately from the other H3 genes that are in the  histone gene cluster on chromosome 6p22-p21.3.

Alternative Names

H3.4;H3/g;H3FT;H3t;HIST3H3;Histone H3;HIST1H3A

SOURCE

A synthetic peptide of human Acetyl-Histone H3-K9.

STORAGE

Store at -20 Stable far one year from the date of shipment.  

REACTIVITY

Human, Mouse, Rat, Other (Wide Range)

ISOTYPE

RECOMMENDED ANTIBODY DILIITIONS

Western blotting   1:500-1:2000  

IF/IHC/IP   1:50-1:200 

ChIP   1:20-1:100

APPLICATION

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Western blot analysis of extracts of various cell lines, using Acetyl-Histone  H3-K9 antibody at 1:1000 dilution.HeLa cells and NIH/3T3 cells and C6  cells were treated by TSA (1 uM) at 37 for 18 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25ug per lane.

T97255

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