Histones are basic nuclear proteins that are responsible for the nucleosome structure  of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp  of DNA wrapped around a histone octamer composed of pairs of each of the four core  histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through  the interaction of a linker histone, H1, with the DNA between the nucleosomes to  form higher order chromatin structures. This gene is intronless and encodes a  replicationdependent histone that is a member of the histone H3 family. Transcripts  from this gene lack polyA tails; instead, they contain a palindromic termination  element. This gene is located separately from the other H3 genes that are in the  histone gene cluster on chromosome 6p22-p21.3.

H3.4;H3/g;H3FT;H3t;HIST3H3;Histone H3;HIST1H3A

A synthetic peptide of human Acetyl-Histone H3-K9.

Rabbit lgG

Human, Mouse, Rat, Other (Wide Range)

Western blot analysis of extracts of various cell lines, using Acetyl-Histone  H3-K9 antibody at 1:1000 dilution.HeLa cells and NIH/3T3 cells and C6  cells were treated by TSA (1 uM) at 37℃ for 18 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25ug per lane.

Western blotting   1:500-1:2000  

IF/IHC/IP   1:50-1:200 

ChIP   1:20-1:100

Store at -20℃ Stable far one year from the date of shipment.