Dioxygenase that demethylates RNA by oxidative demethylation: specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes. Can also demethylate N(6)-methyladenosine in single-stranded DNA (in vitro). Requires molecular oxygen, alpha-ketoglutarate and iron. Demethylation of m6A mRNA affects mRNA processing and export. Required for the late meiotic and haploid phases of spermatogenesis by mediating m6A demethylation in spermatocytes and round spermatids: m6A demethylation of target transcripts is required for correct splicing and the production of longer 3'-UTR mRNAs in male germ cells (By similarity).

RNA demethylase ALKBH5 (EC 1.14.11.53) (Alkylated DNA repair protein alkB homolog 5) (Alpha-ketoglutarate-dependent dioxygenase alkB homolog 5) ALKBH5 ABH5 OFOXD1

Human, Mouse, Rat

WB,IP

WB 1:500-1:2000, IHC 1:50-1:200; IP 1:50-1:100

44KD

ALKBH5 ABH5 OFOXD1

Q6P6C2

https://www.uniprot.org/uniprotkb/q6p6c2

Mouse

Monoclonal

Mouse IgG

Nucleus localization

Differentiation #mRNA processing #Translation regulation #Spermatogenesis

PBS, pH 7.4, 40% glycerol, and 0.01% sodium azide.

Store at -20 °C. Stable for 12 months from date of receipt.