Dioxygenase that mediates demethylation of DNA and RNA containing 1-methyladenosine (m1A). Repairs alkylated DNA containing 1-methyladenosine (m1A) and 3-methylcytosine (m3C) by oxidative demethylation. Has a strong preference for single-stranded DNA. Able to process alkylated m3C within double-stranded regions via its interaction with ASCC3, which promotes DNA unwinding to generate single-stranded substrate needed for ALKBH3. Also acts on RNA. Demethylates N(1)-methyladenosine (m1A) RNA, an epigenetic internal modification of messenger RNAs (mRNAs) highly enriched within 5'-untranslated regions (UTRs) and in the vicinity of start codons. Requires molecular oxygen, alpha-ketoglutarate and iron.

Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3 (EC 1.14.11.33) (EC 1.14.11.54) (Alkylated DNA repair protein alkB homolog 3) (hABH3) (DEPC-1) (Prostate cancer antigen 1) ALKBH3 ABH3 DEPC1

Human, Mouse, Rat

WB,IP,IF

WB 1:500-1:2000, IHC 1:50-1:200; IP 1:50-1:100

32KD

ALKBH3 ABH3 DEPC1

Q96Q83

https://www.uniprot.org/uniprotkb/q96q83

Mouse

Monoclonal

Mouse IgG

DNA excision

PBS, pH 7.4, 40% glycerol, and 0.01% sodium azide.

Store at -20 °C. Stable for 12 months from date of receipt.