Lysine acetylation is an important reversible modification controlling protein activity In various species, acetylation of lysine residues is an important form of protein posttranslational modification(PTM)that affects thousands of proteins such as Histones, transcript factors, IFN receptors, as well as P53, tubulin, Hsp9o that ranging from nuclear regulators to cytosolic proteins, mitochondrial enzymes, and plasma membrane associated receptors(1) The regulation of protein acetylation is involved in control of cell cycle, metabolism, cell signaling, nuclear transport and even other PTM process (1, 2, 3) [1]: Xiang-jiao Y et al. (2008)Molecular Cell 31, 449-461 [2]: Choudhary C et al. (2014) Nature Reviews Molecular Cell Biology 15, 536-550 [3]: Choudhary, C et al. (2009) Science 325. 834-40
Immunoprecipitation >10oug antibody with 5oul Cnbr-sepharose 4B/Protein A Sepharose beads Western Blot 1:200-1:1000
Fig 1 Western blot analysis of chemical acetylated 293T tot Fig2 Relative distribution of acetylated proteins with lysate(Ioug lane 1) un-acetylated 293T total lysate (10 g, lane diverse biological process identified from an immun 2), chemical acetylated BSA (5ng, lane 3) and un-acetylated precipitation LC-MS/MS experiment of mouse liver BSA(5ng, lane 4). using Abmart Acetylated-lysine Mab at a tissue using Acetylated-lysine Mab 1: 1000 dilution Additional information Please add Milli-q water or Abmart solution buffer to the tube before use, at the concentration ofl mg/ml O 2016 Abmart(Shanghai)Co, Ltd
Target Acetylated-lvsine Host Reactivity All spe Isotype Mouse lag
Store at -20°C long term.
AB_2936273
Note: Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.
注意:抗体应用为IHC的,抗体只适合于石蜡切片的组化染色或者荧光染色。
抗体应用为IF/ICC的,抗体适合于冰冻切片的组化染色或者荧光染色,以及细胞水平的化学染色和荧光染色。
艾比玛特医药科技(上海)有限公司
上海市徐汇区桂平路333号聚科生物园区1号楼1-3层
邮箱:market@ab-mart.com
应聘职位:hr@ab-mart.com
订购专线:4006-123-828
销售电话:13162017139(微信同号)
技术支持:13162477137(微信同号)
总机:021-34695901
经销商:QQ 402772198
南方经销商负责:手机13122837132(微信同号)
北方及西南经销商负责:手机13122150513(微信同号)
微信客服
邮箱:market@ab-mart.com
应聘职位:hr@ab-mart.com
订购专线:4006-123-828
销售电话:13162017139(微信同号)
技术支持:13162477137(微信同号)
总机:021-34695901
经销商:QQ 402772198
南方经销商负责:手机13122837132(微信同号)
北方及西南经销商负责:手机13122150513(微信同号)
微信客服
沪ICP备17056956号-2 艾比玛特医药科技(上海)有限公司