TNFSF10, Apo2 Ligand, TL2, Apo2L, CD253

Measure by its ability to induce cytotoxicity in L929 cells in the presence of actinomycin D.
The ED50 for this effect is <1 ng/mL.

TRAIL acts as a cytotoxic protein, through activating rapid apoptosis in tumor cells (not in normal cells). TRAIL conducts apoptosis through binding to DR4 and DR5, which are two death-signaling receptors belonging to the TNFR superfamily of transmembrane proteins. These receptor contain a cytoplasmic "death domain", which can initiate the cell's apoptotic process.

P50592.1

22035

MPRGGRPQKVAAHITGITRRSNSALIPISKDGKTLGQKIESWESSRKGHSFLNHVLFRNGELVIEQEGLYYIYSQTYFRFQEAEDASKMVSKDKVRTKQLVQYIYKYTSYPDPIVLMKSARNSCWSRDAEYGLYSIYQGGLFELKKNDRIFVSVTNEHLMDLDQEASFFGAFLIN with polyhistidine tag at the C-terminus.

His-tag?at?the?C-terminus

Mouse

Mouse

Escherichia coli

E. coli

>98% as determined by SDS-PAGE. Ni-NTA chromatography

<0.1 EU per 1 μg of the protein by the LAL method.

21 kDa

The protein was lyophilized from a solution containing 1X PBS, pH 7.4.

It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

Blue Ice

Lyophilized protein should be stored at -20°C for 1 year.
Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1%
BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months.

Cytokines

Cell culture, Elisa