细胞因子-Recombinant CXCL3 (C-X-C motif chemokine 3), Human ,AF

Datasheet

Synonym/Alternative name(s)

GRO-γ: MGSAγ, MIP-2β, GRO3

Activity

Measure by its ability to chemoattract BaF3 cells transfected with human CXCR2.
The ED50 for this effect is <2 ng/mL.

Protein Description

CXCL3 is an ELR CXC chemokine. Its structural and functional characteristics are similarto GRO1 (CXCL1), GRO2 (CXCL2), and interleukin-8 (CXCL8). CXC chemokines is critical in the phase I inflammation, in which the PMN cells are rapidly chemoattracted. In the next phase of inflammation, the CC chemokines (MCPs) attract different cell subpopulations such as T cells, monocytes, basophils, and eosinophils. MMP12, primarily released by macrophages, can modulate the activity of ELR-CXC chemokines and cleavage human CXCL1, CXCL2 and CXCL3 within the ELR sequence at Glu6-Leu7.

Protein Accession

P19876

Gene ID

2921

Application

Expression Region

ASVVTELRCQCLQTLQGIHLKNIQSVNVRSPGPHCAQTEVIATLKNGKKACLNPASPMVQKIIEKILNKGSTN with polyhistidine tag at the N-terminus.

Tag

His-tag?at?the?N-terminus

Species

Human

Reactivity

Human

Expression Host

Escherichia coli

Source

E. coli

Purification

>98% as determined by SDS-PAGE. Ni-NTA chromatography

Endotoxin

<0.1 EU per 1 μg of the protein by the LAL method.

Calculated Molecular Weight

8.67 kDa

Formulation

The protein was lyophilized from a solution containing 1X PBS, pH 7.4.

Reconstitution

It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

Shipping

Blue Ice

Stability and Storage

Lyophilized protein should be stored at -20°C for 1 year.
Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1%
BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months.

Category

Cytokines

Application

Cell culture, Elisa

Image

SDS- PAGE analysis of recombinant human CXCL3

RMCH054

货号 规格 价格(¥)
RMCH054S 5ug
RMCH054M 20ug
RMCH054L 100ug
RMCH054H 500ug

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Note:  Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.

注意:抗体应用为IHC的,抗体只适合于石蜡切片的组化染色或者荧光染色。

抗体应用为IF/ICC的,抗体适合于冰冻切片的组化染色或者荧光染色,以及细胞水平的化学染色和荧光染色。

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应聘职位:hr@ab-mart.com

订购专线:4006-123-828

销售电话:13162017139(微信同号)

技术支持:13162477137(微信同号)

总机:021-34695901

经销商:QQ 402772198
南方经销商负责:手机13122837132(微信同号)          
北方及西南经销商负责:手机13122150513(微信同号)

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