Platelet Factor-4,PF-4, Oncostatin A, Ironplact
Measure by its ability to inhibit human FGF-2-induce proliferation in HUVEC cells.
The ED50 for this effect is <5 μg/mL.
CXCL4, also known as platelet factor 4 (PF-4), is one of the most plentiful platelet chemokines.Depending on the cell type, CSCL4 may has several biological functions. CXCL4 is mainly produced in megakaryocytes, released from the α-granules of platelets as a tetramer at micromolar concentrations depending on platelet activation. CXCL4 has both procoagulant and anticoagulant activities, thereby can bind heparin and neutralize the anticoagulant effect of heparin. In addition, CXCL4 also have functions such as inhibiting factor XII, and vitamin K dependent coagulation factor, and stimulating activated protein C generation. As a strong tumor inhibitor, CXCL4 can inhibit endothelial cell migration, proliferation, and in vivo angiogenesis through interfering with the angiogenic effect of growth factors such as FGF and VEGF.
P02776.2
5196
EAEEDGDLQCLCVKTTSQVRPRHITSLEVIKAGPHCPTAQLIATLKNGRKICLDLQAPLYKKIIKKLLES with polyhistidine tag at the N-terminus.
His-tag?at?the?N-terminus
Human
Human
Escherichia coli
E. coli
>98% as determined by SDS-PAGE. Ni-NTA chromatography
<0.1 EU per 1 μg of the protein by the LAL method.
8.58 kDa
The protein was lyophilized from a solution containing 1X PBS, pH 7.4.
It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Blue Ice
Lyophilized protein should be stored at -20°C for 1 year.
Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1%
BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months.
Cytokines
Cell culture, Elisa
SDS- PAGE analysis of recombinant human CXCL4 |
Note: Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.
注意:抗体应用为IHC的,抗体只适合于石蜡切片的组化染色或者荧光染色。
抗体应用为IF/ICC的,抗体适合于冰冻切片的组化染色或者荧光染色,以及细胞水平的化学染色和荧光染色。
艾比玛特医药科技(上海)有限公司
上海市徐汇区桂平路333号聚科生物园区1号楼1-3层
邮箱:market@ab-mart.com
应聘职位:hr@ab-mart.com
订购专线:4006-123-828
销售电话:13162017139(微信同号)
技术支持:13162477137(微信同号)
总机:021-34695901
经销商:QQ 402772198
南方经销商负责:手机13122837132(微信同号)
北方及西南经销商负责:手机13122150513(微信同号)
微信客服
邮箱:market@ab-mart.com
应聘职位:hr@ab-mart.com
订购专线:4006-123-828
销售电话:13162017139(微信同号)
技术支持:13162477137(微信同号)
总机:021-34695901
经销商:QQ 402772198
南方经销商负责:手机13122837132(微信同号)
北方及西南经销商负责:手机13122150513(微信同号)
微信客服
沪ICP备17056956号-2 艾比玛特医药科技(上海)有限公司