Granulocyte Chemotactic Protein-2,GCP-2

Measure by its ability to chemoattract BaF3 cells transfected with human CXCR2.
The ED50 for this effect is <10 ng/mL.

CXCL6, also named as GCP-2 in humanspecies, is a CXC chemokine derived from connective tissue. This protein can signal through the CXCR1 and CXCR2 receptors. GCP-2 is a selectively attractant of neutrophils and can also utilize anti-angiogenic activity. Human GCP-2 is found to have cross-reactivity in murine cells assay.

P80162

6372

VSAVLTELRCTCLRVTLRVNPKTIGKLQVFPAGPQCSKVEVVASLKNGKQVCLDPEAPFLKKVIQKILDSGNKKN with polyhistidine tag at the N-terminus.

His-tag?at?the?N-terminus

Human

Human

Escherichia coli

E. coli

>98% as determined by SDS-PAGE. Ni-NTA chromatography

<0.1 EU per 1 μg of the protein by the LAL method.

8.97 kDa

The protein was lyophilized from a solution containing 1X PBS, pH 7.4.

It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

Blue Ice

Lyophilized protein should be stored at -20°C for 1 year.
Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1%
BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months.

Cytokines

Cell culture, Elisa