细胞因子-Recombinant HMGB1 C23AC45A,Human (mammalian cell expression),AF

Datasheet

Synonym/Alternative name(s)

HMG-1, HMG1, HMG3, SBP-1

Activity

Measure by its ability to induce TNF alpha in RAW264.7 cells. The ED50 for this effect is <10 μg/mL.

Protein Description

HMGB1 is present in the nuclei (chromatin associated) and cytoplasm of all cells and is a highly conserved protein in variety of species that. In the cytoplasm, HMGB1 is a regulator of autophagy, enhances cell survival, and limits apoptosis. It also can reduces protein aggregation caused by heat or chemical stress. HMGB1 is released to the extracellular milieu by inflammatory cells and by necrotic and apoptotic cells. Once released, it works as an inflammatory cytokine.HMGB1 is also secreted by macrophages and monocytes as a late response to LPS, TNF-α, IL-1β, or IFN-γ.

Protein Accession

P09429.3

Gene ID

3146

Application

Expression Region

MGKGDPKKPRGKMSSYAFFVQTAREEHKKKHPDASVNFSEFSKKASERWKTMSAKEKGKFEDMAKADKARYEREMKTYIPPKGETKKKFKDPNAPKRPPSAFFLFCSEYRPKIKGEHPGLSIGDVAKKLGEMWNNTAADDKQPYEKKAAKLKEKYEKDIAAYRAKGKPDAAKKGVVKAEKSKKKKEEEEDEEDEEDEEEEEDEEDEDEEEDDDDE with polyhistidine-SUMO tag at the N-terminus

Tag

His-SUMO tag at the N-terminus

Species

Human

Reactivity

Human

Expression Host

HEK293

Source

HEK293

Purification

>98% as determined by SDS-PAGE. Ni-NTA chromatography

Endotoxin

<0.1 EU per 1 μg of the protein by the LAL method.

Calculated Molecular Weight

36.36 kDa

Formulation

The protein was lyophilized from a solution containing 1X PBS, pH 7.4.

Reconstitution

It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

Shipping

Blue Ice

Stability and Storage

Lyophilized protein should be stored at -20°C for 1 year.
Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1%
BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months.

Category

Cytokines

Application

Cell culture, Elisa

Image

SDS- PAGE analysis of recombinant human HMGB1 C23AC45A

RMCH176

货号 规格 价格(¥)
RMCH176S 5ug
RMCH176M 20ug
RMCH176L 100ug
RMCH176H 500ug

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Note:  Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.

注意:抗体应用为IHC的,抗体只适合于石蜡切片的组化染色或者荧光染色。

抗体应用为IF/ICC的,抗体适合于冰冻切片的组化染色或者荧光染色,以及细胞水平的化学染色和荧光染色。

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应聘职位:hr@ab-mart.com

订购专线:4006-123-828

销售电话:13162017139(微信同号)

技术支持:13162477137(微信同号)

总机:021-34695901

经销商:QQ 402772198
南方经销商负责:手机13122837132(微信同号)          
北方及西南经销商负责:手机13122150513(微信同号)

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