Plasmid vectors for the expression of coding regions of eukaryotic genes in bacterial, insect and mammalian hosts are in common usage; such expression vectors are frequently used to encode hybrid fusion proteins consisting of a eukaryotic target protein and a specialized region designed to aid in the purification and visualization of the target protein. For example, the pCDM8 expression vector and derivatives thereof encode fusions between the target protein and an 11 amino acid peptide derived from the influenza protein hemagglutinin (HA). The HA epitope tag is useful in Western blotting and immunohistochemical localization of expressed fusion proteins when examined with antibodies raised specifically against the HA-epitope tag.
Mouse
Mouse IgG1
HA-Tag (26D11) Mouse mAb detects over-expressed proteins containing the HA epitope tag.
All
Western blotting 1:5000
Immunofluorescence 1:1000
Immunoprecipitation 1:100
ELISA 1:2000
Store at -20°C. Stable for one year from the date of shipment.
AB_2936257
1. Hopp, T.P., Prickett, K.S., Price, V.L., Libby, R.T., March, C.J., Cerretti, D.P.,Urdal, D.L. and Conlon, P.J. 1988. A short polypeptide marker sequence useful for recombinant protein identification and purification. Nat. Biotechnol. 6: 1204-1210.
2. Smith, D.B. and Johnson, K.S. 1988. Single-step purification of polypeptides expressed in Escherichia coli as fusions with Glutathione S-transferase.Gene 67: 31-40.
#M20021 Anti-Ha-Tag Antibody (HRP Conjugated)
#M20022 Anti-GFP-Tag Antibody(HRP Conjugated)
#M20023 β-Tubulin Antibody(HRP Conjugated)
#M20024 GAPDH (3B3)Antibody(HRP Conjugated)
#M20025 Anti-GST-Tag Antibody(HRPConjugated)
#M20026 Anti-DYKDDDDK-Tag Antibody(HRPConjugated)
Note: Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.
注意:抗体应用为IHC的,抗体只适合于石蜡切片的组化染色或者荧光染色。
抗体应用为IF/ICC的,抗体适合于冰冻切片的组化染色或者荧光染色,以及细胞水平的化学染色和荧光染色。
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邮箱:market@ab-mart.com
应聘职位:hr@ab-mart.com
订购专线:4006-123-828
销售电话:13162017139(微信同号)
技术支持:13162477137(微信同号)
总机:021-34695901
经销商:QQ 402772198
南方经销商负责:手机13122837132(微信同号)
北方及西南经销商负责:手机13122150513(微信同号)
微信客服
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