标签/内参抗体-Anti-GST-Tag Antibody(HRPConjugated)

Datasheet

Background

Plasmid vectors for the expression of coding regions of eukaryotic genes in E.coli are in common usage; such expression vectors often encode hybrid fusion proteins containing part prokaryotic and part eukaryotic specified proteins. For instance, the pGEX.3X expression vector developed by Smith and Johnson allows for synthesis of fusion proteins between glutathionine-S-transferase
(GST) and proteins encoded by inserted cDNA sequences. Antibodies derived from these GST fusion proteins are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in E. Coli.

Source

Mouse

Isotype

Mouse IgG1

Immunogen

This Abmart monoclonal antibody is produced by immunizing animals with GST.

Specificity

GST-Tag (12G8) Mouse mAb detects GST and GST fusion proteins.

Application

Reactivity

All

Recommanded Dilutions

Western blotting 1:5000
Immunofluorescence   1:1000
Immunoprecipitation     1:100
ELISA                                1:2000

Storage

Store at -20°C. Stable for one year from the date of shipment.

RRID

AB_2936260

References

1.Maniatis, T., Fritsch, E.F. and Sambrook, J. 1982. Molecular Cloning- A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
2. Smith, D.B. and Johnson, K.S. 1988. Single-step purification of polypeptides expressed in Escherichia coli as fusions with Glutathione S-transferase. Gene 67: 31-40.
3. Crabb, B.S. and Studdert, M.J. 1995. Expression of small regions of equine herpesvirus 1 glycoprotein C in Escherichia coli. Vet. Microbiol. 46: 181-191.
4. Soler, D., Nomizu, T., Brown, W.E., Shibata, Y. and Auld, D.S. 1995. Matrilysin: expression, purification, and characterization. J. Protein Chem.14: 511-520.

Companion Products

#M20021 Anti-Ha-Tag Antibody (HRP Conjugated)
#M20022 Anti-GFP-Tag Antibody(HRP Conjugated)
#M20023 β-Tubulin Antibody(HRP Conjugated)
#M20024 GAPDH (3B3)Antibody(HRP Conjugated)
#M20025 Anti-GST-Tag Antibody(HRPConjugated)
#M20026 Anti-DYKDDDDK-Tag Antibody(HRPConjugated)

M20025

货号 规格 价格(¥)
M20025H 1000μl
M20025L 200μl
M20025M 100μl
M20025S 50μl

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Note:  Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.

注意:抗体应用为IHC的,抗体只适合于石蜡切片的组化染色或者荧光染色。

抗体应用为IF/ICC的,抗体适合于冰冻切片的组化染色或者荧光染色,以及细胞水平的化学染色和荧光染色。

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邮箱:market@ab-mart.com

应聘职位:hr@ab-mart.com

订购专线:4006-123-828

销售电话:13162017139(微信同号)

技术支持:13162477137(微信同号)

总机:021-34695901

经销商:QQ 402772198
南方经销商负责:手机13122837132(微信同号)          
北方及西南经销商负责:手机13122150513(微信同号)

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