A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine-tag, 6xHis-tag, and by the trademarked name His-tag. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; His tag

Mouse

Monoclonal Antibody

IgG1

Synthetic peptide: HHHHHH conjugated to KLH.

Transfected

WB: 1/1000-1/10000 IF: 1/100-1/500 IP: 1/20

Refer to figures

Affinity Purified

Liquid

1 mg/ml

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Store at 4°C short term. Store at -20°C long term. Avoid freeze/thaw cycles.