The green fluorescent protein (GFP) was originally identified as a protein involved in the bioluminescence of the jellyfish Aequorea victoria. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors, making GFP a useful tool for monitoring gene expression and protein localization in vivo. Several GFP mutants have been developed, including EGFP, which fluoresce more intensely than the wildtype GFP and have shifted excitation maxima, making them useful  for FACS and fluorescence microscopy as well as double-labeling applications. GFP is widely used in expression vectors as a fusion protein tag, allowing expression and monitoring of heterologous proteins fused to GFP.

Rabbit

Rabbit IgG

This Abmart polyclonal antibody is produced by immunizing animals with a synthetic  peptide (KLH-coupled) corresponding to the carboxy terminus of EGFP.

GFP Antibody detects GFP and  GFP fusion proteins.

All

Western blotting                   1:2000
Immunoprecipitation           1:100

Store at -20°C. Stable for one year from the date of shipment.

AB_2936507

1. Prasher, D.C., et al. 1992. Primary structure of the Aequorea victoria green fluorescent protein. Gene 111: 229-233.
2. Chalfie, M., et al. 1994. Green fluorescent protein as a marker for gene expression. Science 263: 802-805.

#M20001 His-Tag (2A8) Mouse mAb
#M20002  Myc-Tag  (19C2) Mouse mAb
#M20003  HA-Tag (26D11) Mouse mAb
#M20004 GFP-Tag (7G9) Mouse mAb
#M20007  GST-Tag (12G8) Mouse mAb
#M20008  DYDDDDDK-Tag (3B9) Mouse mAb (Binds to same epitope as Sigma’s Anti-FLAG? M2 Antibody)
#M20012 Anti-Myc-Tag Mouse mAb (Agarose Conjugated)
#M20013 Anti-HA-Tag Mouse mAb (Agarose Conjugated)
#M20018 Anti-DYKDDDDK-Tag Mouse mAb (Agarose Conjugated) (Binds to same epitope as Sigma’s Anti-FLAG? M2 Antibody)
#M21002 Goat Anti-Rabbit IgG-HRP