Beads抗体-Anti-GFP-Tag Antibody (Agarose conjugated)

Datasheet

Background

The green fluorescent protein (GFP) was originally identified as a protein involved in the bioluminescence of the jellyfish Aequorea victoria. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors, making GFP a useful tool for monitoring gene expression and protein localization in vivo. Several GFP mutants have been developed, including EGFP, which fluoresce more intensely than the wildtype GFP and have shifted excitation maxima, making them useful for FACS and fluorescence microscopy as well as double-labeling applications. GFP is widely used in expression vectors as a fusion protein tag, allowing expression and monitoring of heterologous proteins fused to GFP.

Source

This Abmart monoclonal antibody is produced by immunizing animals with full length recombinant GFP.

References

1. Prasher, D.C., et al. 1992. Primary structure of the Aequorea victoria green fluorescent protein. Gene 111: 229-233.
2. Chalfie, M., et al. 1994. Green fluorescent protein as a marker for gene expression. Science 263: 802-805.

Specificity

GFP-Tag (7G9) Mouse mAb detects GFP and GFP fusion proteins.

Storage

Theproductissuppliedasa50%slurryinstoragebuffer(1PBS,pH7.4,containing0.1%NaN).

Storetheproductat4°Canddonotfreeze.

Application

Reactivity

All

Isotype

Mouse IgG1

M20014

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