二抗-Goat anti-Mouse IgG UltraPolymer antibody(HRP-conjugated)

Datasheet

Description

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. Horseradish peroxidase (HRP) conjugates are prepared by a modified Nakane and Kawaoi procedure (J. Histochem. Cytochem. 1974. 22, 1084). Peroxidase conjugates are commonly used for immunohistochemistry, Western blotting, and ELISA. Affinity-purified anti-horseradish peroxidase and conjugates are available for detection of horseradish peroxidase antigen or for signal amplification of HRP-containing reagents. For immunostaining of mammalian cells, an advantage of using anti-horseradish peroxidase is reduced background, since the antibody does not recognize the endogenous peroxidase-like enzymes found in those cells.
UltraPolymer Goat anti-Mouse IgG (H&L) conjugated to HRP, affinity purified, min x w/ bovine, horse, human, pig or rabbit serum proteins.

Research areas

Uniprot ID

Molecular Wt.

Species reactivity

Mouse

Application

Predicted species reactivity

Antibody type

Goat polyclonal Antibody

Isotype

Goat IgG

Storage buffer

TBS, 1% (w/v) BSA, 0.1% Proclin 150, 10% heat-inactivated animal serum.

Form

Liquid

Concentration

Ready to Use.

Purity

Affinity purified using solid phase Mouse IgG.

Alternative names

Positive control

Subcellular location

Recommended dilutions

IHC-P: Ready to Use

Storage

Store at 2-8 ℃.

GU101120

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