Recruited by UPF3 associated with the EJC core at the cytoplasmic side of the nuclear envelope and the subsequent formation of an UPF1-UPF2-UPF3 surveillance complex (including UPF1 bound to release factors at the stalled ribosome) is believed to activate NMD. In cooperation with UPF3 stimulates both ATPase and RNA helicase activities of UPF1. Binds spliced mRNA (By similarity). Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by associating with the nuclear exon junction complex (EJC). Required for plant development and adaptation to environmental stresses, including plant defense and response to wounding.

Regulator of nonsense transcripts UPF2; Nonsense mRNA reducing factor UPF2; Up-frameshift suppressor 2 homolog; At2g39260;

F4IUX6

134  kDa

Mouse

Arabidopsis thaliana

Solanum tuberosum; Capsicum annuum; Arabidopsis thaliana; Oryza sativa; Malus domestica; Prunus persica; Rosa chinensis; Gossypium mustelinum; 

 WB 1:500-1:2000, IHC 1:50-1:200, IP: 1:50-1:200

Figure 2. UPF1, UPF2, and UPF3 Decay during an Early Phase of PstDC3000 Infection.

(A) Dynamics of UPF1, UPF2, and UPF3 proteins up to 30 hpi (top) and 50 mpi (bottom). Immunoblot analyses (left) were performed for leaf samples taken from wild-type Col-0 plants that had been infected with Pseudomonas and collected at the indicated time points using an anti-UPF1 monoclonal antibody (a-UPF1), a-UPF2, ora-UPF3. The right shows UPF protein levels in infected leaves at the indicated time points (average6SD, n54).Different letters above the bars (i.e., a, b, c, d, ab, and bc) indicate statistically significant differences (P < 0.05, one-way ANOVA).Successful infection was verified by examining the levels of PR1. Both experiments were performed with four biological replicates.

(B) TheNMDfactorSMG7and the EJC core protein components, but not UPF1, UPF2, or UPF3, remained stable during PstDC3000 infection. The leaves of transgenic Arabidopsis plants (T3) stably expressing protein A-fused UPF1, UPF2, UPF3, SMG7, Y14, MAGO, elF4A-III, BTZ1, and BTZ2 were infected with PstDC3000 and collected at the indicated time points after infection for immunoblot analysis. GFP-protein A was used as a representative stable protein. The recombinant proteins were detected using an a-PAP antibody. M, prestained protein ladder.

References: Pathogen-Associated Molecular Pattern-Triggered Immunity Involves Proteolytic Degradation of Core Nonsense-Mediated mRNA Decay Factors During the Early Defense Response. The Plant Cell, Vol. 32: 1081–1101, April 2020

Store at -20 °C. Stable for 12 months from date of receipt.