标签/内参抗体-GAPDH mAb

Datasheet

Background

Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively abundant expressed in almost cell types at high levels, therefore antibodies against GAPDH are useful as loading controls for Western Blotting. Some pathology factors, such as hypoxia and diabetes, increased or decreased GAPDH expression in certain cell types.

Gene Name

GAPDH

Alternative Names

G3PD; GAPD; HEL-S-162eP

Uniprot

P04406

Source

Mouse

Application

Clonality

Monoclonal Antibody

Isotype

IgG1

Immunogen

Synthetic Peptide of GAPDH

Reactivity

Human,Rat,Mouse,Monkey,Dog,Chicken,Hamster,Rabbit,Pig,Sheep,Insect,Yeast

Dilution

WB: 1/5000-1/10000 IHC: 1/50-1/200 IF: 1/100-1/200

Molecular Weight

Calculated MW: 36 kDa; Observed MW: 36 kDa

Conjugation

Purification

Affinity Purified

Modification

Form

Liquid

Concentration

2.5 mg/ml

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Storage

Store at 4°C short term. Store at -20°C long term. Avoid freeze/thaw cycles.

MN50133

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MN50133S 50ul
MN50133M 100ul

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Note:  Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.

注意:抗体应用为IHC的,抗体只适合于石蜡切片的组化染色或者荧光染色。

抗体应用为IF/ICC的,抗体适合于冰冻切片的组化染色或者荧光染色,以及细胞水平的化学染色和荧光染色。

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