Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion.

DYKDDDDK Tag; Flag; FLAG

Mouse

Monoclonal Antibody

IgG1

Synthetic peptide conjugated to KLH.

Transfected

WB: 1/1000-1/10000

Refer to figures

Affinity Purified

Liquid

1 mg/ml

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Store at 4°C short term. Store at -20°C long term. Avoid freeze/thaw cycles.