Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties. The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems.

Mouse IgG1

This Abmart monoclonal antibody is produced by immunizing animals with a synthetic peptide (KLH-coupled) corresponding to residues 410-419 of human c-Myc (EQKLISEEDL).

Myc-Tag (19C2) Mouse mAb detects over-expressed or recombinant proteins containing the Myc epitope tag.

All

Western blotting            1:5000
Immunofluorescence   1:2000
Immunoprecipitation     1:200
ELISA                                1:2000

Store at -20°C. Stable for one year from the date of shipment.

AB_2861172

Munro, S. and Pelham, H.R. 1984. Use of peptide tagging to detect proteins expressed from cloned genes: deletion mapping functional domains of Drosophila hsp 70. EMBO J. 3, 3087-3093.

#M20001 His-Tag (2A8) Mouse mAb
#M20003 HA-Tag (26D11) Mouse mAb
#M20004 GFP-Tag (7G9) Mouse mAb
#M20007 GST-Tag (12G8) Mouse mAb
#M20008 DYDDDDDK-Tag (3B9) Mouse mAb (Binds to same epitope as Sigma’s Anti-FLAG M2 Antibody)
#M20012 Anti-Myc-Tag Mouse mAb (Agarose Conjugated)
#M20013 Anti-HA-Tag Mouse mAb (Agarose Conjugated)
#M20018 Anti-DYKDDDDK-Tag Mouse mAb (Agarose Conjugated) (Binds to same epitope as Sigma’s Anti-FLAG M2 Antibody)