Plasmid vectors for the expression of coding regions of eukaryotic genes in bacterial, insect and mammalian hosts are in common usage; such expression vectors are frequently used to encode hybrid fusion proteins consisting of a eukaryotic target protein and a specialized region designed to aid in the purification and visualization of the target protein. For example, the pCDM8 expression vector and derivatives thereof encode fusions between the target protein and an 11 amino acid peptide derived from the influenza protein hemagglutinin (HA). The HA epitope tag is useful in Western blotting and immunohistochemical localization of expressed fusion proteins when examined with antibodies raised specifically against the HA-epitope tag.

Mouse IgG1

This Abmart monoclonal antibody is produced by immunizing animals with a synthetic peptide containing the infuenza hemagglutinin epitope (YPYDVPDYA) (KLH-coupled).

HA-Tag (26D11) Mouse mAb detects over-expressed proteins containing the HA epitope tag.

All

Western blotting            1:5000
Immunofluorescence   1:1000
Immunoprecipitation     1:100
ELISA                                1:2000

Store at -20°C. Stable for one year from the date of shipment.

AB_2864345

1. Hopp, T.P., Prickett, K.S., Price, V.L., Libby, R.T., March, C.J., Cerretti, D.P.,Urdal, D.L. and Conlon, P.J. 1988. A short polypeptide marker sequence useful for recombinant protein identification and purification. Nat. Biotechnol. 6: 1204-1210.
2. Smith, D.B. and Johnson, K.S. 1988. Single-step purification of polypeptides expressed in Escherichia coli as fusions with Glutathione S-transferase.Gene 67: 31-40.

#M20001 His-Tag (2A8) Mouse mAb
#M20002 Myc-Tag (19C2) Mouse mAb
#M20004 GFP-Tag (7G9) Mouse mAb
#M20007 GST-Tag (12G8) Mouse mAb
#M20008 DYDDDDDK-Tag (3B9) Mouse mAb (Binds to same epitope as Sigma’s Anti-FLAG M2 Antibody)
#M20012 Anti-Myc-Tag Mouse mAb (Agarose Conjugated)
#M20013 Anti-HA-Tag Mouse mAb (Agarose Conjugated)
#M20018 Anti-DYKDDDDK-Tag Mouse mAb (Agarose Conjugated) (Binds to same epitope as Sigma’s Anti-FLAG M2 Antibody)